Added Colorbar for each slice of scan
improved UI, better response, better bug report, minor fixes
This commit is contained in:
José Julián Sierra Álvarez
GitHub
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2aaa53ee42
commit
1caf0a76c3
26
README.md
26
README.md
@ -1,18 +1,22 @@
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# Julia_imzML_GUI<br />
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A Graphical User Interface created with Julia Genie Builder made for this repository: https://github.com/CINVESTAV-LABI/julia_mzML_imzML that generates images with the imzML and ibd files.<br />
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For this to work you must insert the next lines as they are depicted in your Julia terminal for the first time only: <br />
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# Julia_msi_GUI<br />
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A Graphical User Interface created with <i>Julia Genie Builder</i> made for to assist in the use of this repository: https://github.com/CINVESTAV-LABI/julia_mzML_imzML which generates images with the mzMl, imzML and ibd files.<br />
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For the correct implementation and use of this repository one must insert the next lines just as they are depicted in your <b>Julia</b> terminal to install the dependencies needed, this part is only needed once. <br />
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```
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julia
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]
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add Pkg Libz ; add https://github.com/CINVESTAV-LABI/julia_mzML_imzML ; add PlotlyBase ; add Statistics
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```
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Then open a terminal on the directory "JuliaIMZML_GUI" and put the next code: <br />
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```
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julia
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]
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activate .
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add Pkg Libz ; add https://github.com/CINVESTAV-LABI/julia_mzML_imzML ; add PlotlyBase ; add Statistics ; add CairoMakie ; add Colors ; add
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add Genie
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##(press backspace)
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```
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Then open a <b>Julia</b> terminal on the directory <i>"Julia_msi_GUI"</i> and put the next code, this is needed every time you want to run the GUI: <br />
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```
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]
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```
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to enter Pkg mode.
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```
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activate .
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```
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Press backspace key to exit Pkg mode.
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```
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using Genie ; Genie.loadapp() ; up()
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```
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And finally open the port that gets generated to access to the GUI<br />
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82
app.jl
82
app.jl
@ -1,10 +1,11 @@
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module App
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# == Packages ==
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# set up Genie development environment.
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using GenieFramework
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using GenieFramework # set up Genie development environment.
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using Pkg
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using Libz
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using PlotlyBase
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using CairoMakie
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using Colors
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using julia_mzML_imzML
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using Statistics
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@genietools
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@ -12,9 +13,10 @@ using Statistics
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# == Code import ==
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# add your data analysis code here or in the lib folder. Code in lib/ will be
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# automatically loaded
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rgb_ViridisPalette =reinterpret(ColorTypes.RGB24, ViridisPalette)
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# == Reactive code ==
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# add reactive code to make the UI interactive
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# reactive code to make the UI interactive
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@app begin
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# == Reactive variables ==
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# reactive variables exist in both the Julia backend and the browser with two-way synchronization
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@ -42,14 +44,16 @@ using Statistics
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@in ImgPlusT = false
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@in ImgMinusT = false
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@out testT = "/.bmp"
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@in msg = ""
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@in msgimg = ""
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@in msgtriq = ""
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@out msg = ""
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@out msgimg = ""
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@out msgtriq = ""
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@out full_route = ""
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@out full_routeMz = ""
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@out full_routeMz2 = ""
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@out colorbar = "/.png"
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@out colorbarT = "/.png"
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layoutSpectra = PlotlyBase.Layout(
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title = "Spectra Plot",
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title = "SUM Spectrum plot",
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xaxis = PlotlyBase.attr(
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title = "<i>m/z</i>",
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showgrid = true
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@ -70,11 +74,12 @@ using Statistics
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# its value changes
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@onchange triqEnabled begin
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if !triqEnabled
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triqProb = 0.0
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triqColor = 0
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#triqProb = 0.0
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#triqColor = 0
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end
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end
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@onchange file_name begin
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msg = ""
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if contains(file_name,".imzML")
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warning_fr = ""
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full_route = joinpath( file_route, file_name )
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@ -96,8 +101,11 @@ using Statistics
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Disab_btn = false
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full_routeMz2 = full_routeMz # to avoid creating the plot if its the same file read as before
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end
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else
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warning_fr = "is not an imzML file"
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end
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else
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full_route = "/"
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warning_fr = "is not an imzML file"
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end
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end
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@ -107,38 +115,44 @@ using Statistics
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lastimg = floor(Int, Nmass)
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lastimgTriq = floor(Int, Nmass)
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end
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# the onbutton handler will set the variable to false after the block is executed
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# The onbutton handler will set the variable to false after the block is executed
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@onbutton Main_Process begin
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Disab_btn = true # We disable the button to avoid multiple requests
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indeximg = floor(Int, Nmass)
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full_route = joinpath(file_route, file_name)
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if isfile(full_route) && Nmass > 0 && Tol > 0 && Tol <= 1
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msg = "File exists, Nmass=$(Nmass) Tol=$(Tol). Please do not press the start button until confirmation"
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msg = "File exists, Nmass=$(Nmass) Tol=$(Tol). Loading file will begin, please be patient."
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try
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spectra = LoadImzml(full_route)
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msg = "File loaded. Please do not press the start button until confirmation"
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msg = "File loaded. Creating Spectra with the specific mass and tolerance, please be patient."
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slice = GetSlice(spectra, Nmass, Tol)
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if triqProb != 0 # if we have TrIQ
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if triqColor < 1 || triqColor > 256
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triqColor = 1
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end
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if triqProb < 0 || triqProb > 1
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triqProb = 0.1
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end
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SaveBitmap(joinpath("public", "TrIQ_$(floor(Int, Nmass)).bmp"),
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TrIQ(slice, Int(triqColor), triqProb),
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ViridisPalette)
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fig = CairoMakie.Figure(size = (100, 200)) #container
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if triqEnabled # if we have TrIQ
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if triqColor < 1 || triqColor > 256 ||triqProb < 0 || triqProb > 1
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msg = "Incorrect TrIQ values, please adjust accordingly and try again."
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else
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SaveBitmap(joinpath("public", "TrIQ_$(floor(Int, Nmass)).bmp"),TrIQ(slice, Int(triqColor), triqProb),ViridisPalette)
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testT = "/TrIQ_$(floor(Int, Nmass)).bmp" # we define the starting value of the images
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msgtriq = "TrIQ image with the Nmass of $(floor(Int, Nmass))"
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else # if we don't
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SaveBitmap(joinpath("public", "$(floor(Int, Nmass)).bmp"),
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IntQuant(slice),
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ViridisPalette)
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Colorbar(fig[1, 1], colormap = rgb_ViridisPalette, limits = (0, maximum(TrIQ(slice, Int(triqColor), triqProb))))
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save("public/colorbar_TrIQ_$(floor(Int, Nmass)).png", fig)
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msg = "The file has been created successfully inside the 'public' folder of the app."
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colorbarT = "/colorbar_TrIQ_$(floor(Int, Nmass)).png"
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end
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else # if we don't use TrIQ
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SaveBitmap(joinpath("public", "$(floor(Int, Nmass)).bmp"),IntQuant(slice),ViridisPalette)
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test = "/$(floor(Int, Nmass)).bmp" # we define the starting value of the images
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msgimg = "image with the Nmass of $(floor(Int, Nmass))"
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Colorbar(fig[1, 1], colormap = rgb_ViridisPalette, limits = (0, maximum(slice)))
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save("public/colorbar_$(floor(Int, Nmass)).png", fig)
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msg = "The file has been created successfully inside the 'public' folder of the app."
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colorbar = "/colorbar_$(floor(Int, Nmass)).png"
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end
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catch e
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msg = "There was an error loading the ImzML file, please verify the file accordingly and try again. $(e)"
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end
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msg = "The file has been created inside the 'public' folder of the app"
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else
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msg = "File does not exist or a parameter was not well inputted"
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msg = "File does not exist or a parameter is incorrect, please try again."
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end
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spectra = nothing # Important for memory cleaning
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slice = nothing
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@ -155,10 +169,9 @@ using Statistics
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indeximg = lastimg
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end
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test = "/$(indeximg).bmp"
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colorbar = "/colorbar_$(indeximg).png"
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msgimg = "image with the Nmass of $(indeximg)"
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lastimg = indeximg
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GC.gc() # Trigger garbage collection
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ccall(:malloc_trim, Int32, (Int32,), 0) # Ensure julia returns the freed memory to OS
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end
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@onbutton ImgPlus begin
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indeximg+=1
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@ -169,10 +182,9 @@ using Statistics
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indeximg = lastimg
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end
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test = "/$(indeximg).bmp"
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colorbar = "/colorbar_$(indeximg).png"
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msgimg = "image with the Nmass of $(indeximg)"
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lastimg = indeximg
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GC.gc() # Trigger garbage collection
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ccall(:malloc_trim, Int32, (Int32,), 0) # Ensure julia returns the freed memory to OS
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end
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@onbutton ImgMinusT begin
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@ -184,10 +196,9 @@ using Statistics
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indeximgTriq = lastimgTriq
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end
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testT = "/TrIQ_$(indeximgTriq).bmp"
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colorbarT = "/colorbar_TrIQ_$(indeximgTriq).png"
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msgtriq = "TrIQ image with the Nmass of $(indeximgTriq)"
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lastimgTriq = indeximgTriq
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GC.gc() # Trigger garbage collection
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ccall(:malloc_trim, Int32, (Int32,), 0) # Ensure julia returns the freed memory to OS
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end
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@onbutton ImgPlusT begin
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indeximgTriq+=1
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@ -198,10 +209,9 @@ using Statistics
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indeximgTriq = lastimgTriq
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end
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testT = "/TrIQ_$(indeximgTriq).bmp"
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colorbarT = "/colorbar_TrIQ_$(indeximgTriq).png"
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msgtriq = "TrIQ image with the Nmass of $(indeximgTriq)"
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lastimgTriq = indeximgTriq
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GC.gc() # Trigger garbage collection
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ccall(:malloc_trim, Int32, (Int32,), 0) # Ensure julia returns the freed memory to OS
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end
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GC.gc() # Trigger garbage collection
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ccall(:malloc_trim, Int32, (Int32,), 0) # Ensure julia returns the freed memory to OS
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41
app.jl.html
41
app.jl.html
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<header>
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<h4>Julia mzML imzML GUI </h4>
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<h6>Please make sure the ibd, the mzML and the imzML files are located in the same directory and have the same name</h6>
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<h6>It may take a while to generate the image and the plot, please be patient</h6>
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<header id="ikbk"><!--<img src="/css/biotech_100dp_1B1F3B.png" alt="Bioteck Icon" id="i65e">-->
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<img src="/css/LABI_logo.jpeg" alt="Labi Logo Icon" id="i65e">
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<div>
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<h4>Julia mzML imzML analysis GUI </h4>
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<h6>Please make sure the ibd, the mzML and the imzML files are located in the same directory and have the same name.</h6>
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<h6>It may take a while to generate the image and the plot, please be patient.</h6>
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</div>
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</header>
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<div class="row">
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<div class="st-col col-12 col-sm st-module">
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<div class="st-col col-12 col-sm st-module" id="i0xj8">
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<div class="row">
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<div class="st-col col-12 col-sm st-module">
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<div class="st-col col-12 col-sm st-module" id="idbha">
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<!--Left DIV-->
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<!--<q-file v-model="file_route" filled="" label="Select your imzML file" accept=".imzML"></q-file>-->
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<q-input id="ijii1" v-model="file_route" :dense="true" label="Insert the route to your imzML file"></q-input>
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<p id="i7brk">full route: {{full_route}} {{warning_fr}}</p>
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<div class="row">
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<div class="st-col col-12 col-sm">
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<q-input id="icc9d" v-model="Nmass" :dense="true" label="Mass Value" type="number" :rules="[
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<q-input id="icc9d" v-model="Nmass" :dense="true" label="Mass-to-charge ratio of interest" type="number" :rules="[
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val => val >= 0 || 'Need positive mass values',
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]"></q-input>
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</div>
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<div class="st-col col-12 col-sm">
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<q-input id="iqt3l" step="0.01" v-model="Tol" :dense="true" label="Tolerance" type="number" :rules="[
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<q-input id="iqt3l" step="0.01" v-model="Tol" :dense="true" label="Mass-to-charge ratio Tolerance" type="number" :rules="[
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val => !!val || '* Required',
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val => val >= 0 && val <= 1 || 'Needs to be in range between 0 and 1',
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]"></q-input>
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]" :readonly="!triqEnabled"></q-input>
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</div>
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<div class="st-col col-12 col-sm-4">
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<q-input id="i28wb-2" step="1" v-model="triqColor" :dense="true" label="TrIQ Color" type="number" :rules="[
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<q-input id="i28wb-2" step="1" v-model="triqColor" :dense="true" label="TrIQ Color Levels" type="number" :rules="[
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val => val >= 0 && val <= 256 || 'Needs to be in range between 1 and 256',
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]" :readonly="!triqEnabled"></q-input>
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</div>
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<q-btn id="isfqg" :disabled="Disab_btn" color="primary" icon="play_arrow" v-on:click="Main_Process = true" label="Start"></q-btn>
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<p>{{msg}}</p>
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</div>
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</div><!-- <plotly :data="plotdata" :layout="plotlayout"> </plotly>-->
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</div>
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<!-- <plotly :data="plotdata" :layout="plotlayout"> </plotly>-->
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</div>
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<div class="st-col col-12 col-sm st-module">
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<div class="st-col col-12 col-sm st-module" id="i8pdn">
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<div class="row">
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<div class="st-col col-12 col-sm st-module">
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<div class="st-col col-12 col-sm st-module" id="ihxlc">
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<!--Right DIV-->
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<h6>Image Visualizer</h6><br>
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<p></p>
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<q-btn id="ijhjl-2" color="primary" icon="arrow_back" label="Previous" v-on:click="ImgMinus = true"></q-btn>
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<q-btn id="i3zt1" color="primary" icon="arrow_forward" label="Next" v-on:click="ImgPlus = true"></q-btn>
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<q-img id="i6e3p" :src="test"></q-img>
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<div id="image-container" class="row col-12">
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<div class="col-10"><q-img id="i6e3p" :src="test"></q-img></div>
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<div class="col-2"><q-img id="colorbar" :src="colorbar"></q-img></div>
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</div>
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<p>{{msgimg}}</p>
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<q-btn id="ijhjl-2-2" color="primary" icon="arrow_back" label="Previous" v-on:click="ImgMinusT = true"></q-btn>
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<q-btn id="i3zt1-2" color="primary" icon="arrow_forward" label="Next" v-on:click="ImgPlusT = true"></q-btn>
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<q-img id="i6e3p-2" :src="testT"></q-img>
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<div id="image-container" class="row col-12">
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<div class="col-10"><q-img id="i6e3p-2" :src="testT"></q-img></div>
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<div class="col-2"><q-img id="colorbar-2" :src="colorbarT"></q-img></div>
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</div>
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<p>{{msgtriq}}</p>
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</div>
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</div>
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</div>
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<div class="row">
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<div class="st-col col-12 col-sm st-module">
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<plotly :data="plotdata" :layout="plotlayout"> </plotly>
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<plotly id="iiglk" :data="plotdata" :layout="plotlayout"></plotly>
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</div>
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</div>
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