imzML & mzML Data Pre-Treatment
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Method
Applies a variance-stabilizing transformation to the intensity vector.




Method
The smoothing algorithm.

Parameters
Method
The algorithm to use for baseline correction.


Parameters
Method
The normalization method to apply.



Method
The alignment algorithm.


Parameters
Define Internal Standards / Reference Peaks

Provide m/z values and optional labels for internal standards or reference peaks. These are used for mass calibration and alignment.

This step uses the peaks defined in the 'Internal Standards' tab to correct the m/z axis.

Parameters

Select the appropriate peak picking method and set its parameters.

Method
The peak detection algorithm.


Parameters
Peak Quality Filters
Method
The binning strategy.

Parameters
imzML & mzML Data Processor

Please make sure the ibd and imzML file are located in the same directory and have the same name.
It may take a while to generate the slice / spectrum, please be patient.
To generate the contour or surface plots, you have to select the desired slice first using the interface.

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Mean spectrum plot Sum Spectrum plot Spectrum plot (X,Y)
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Image topography Plot TrIQ topography Plot Image surface Plot TrIQ Surface Plot Over normal image Over TrIQ image
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mzML to imzML Converter

Select the .mzML file and the corresponding .txt synchronization file to convert them into an .imzML/.ibd pair.

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Spectrum View
Before Preprocessing
After Preprocessing
Image visualizer
TrIQ visualizer
Mean spectrum plot Sum Spectrum plot Spectrum plot (X,Y)